Journal: Journal of visualized experiments : JoVE

Article Title: A Model for Epilepsy of Infectious Etiology using Theiler’s Murine Encephalomyelitis Virus

doi: 10.3791/63673

Figure Lengend Snippet: Materials

Article Snippet: 1 mg–220 g Animal balance Ohaus (Parsippany, NJ, U.S.A.) STX2202 0.01 g–2200 g BD Lo-Dose U-100 Insulin Syringes BD (Mississauga, ON, Canada) BD329461 Lo-Dose sterile syringes with permanent BD Micro-Fine IV needle - 1 mL Daniel’s strain of TMEV kindly provided by Robert Fujinami (University of Utah) - 3 x 10 5 plaque-forming units aliquot(s) Disinfectant, e.g. VennoVet 1 super Menno Chemie Vertriebsgesellschaft GmbH, Germany - Recommended by campus veterinarians with less than or equal to 5% alcohol Fisherbrand medium sterile Alcohol prep pad C7 Thermo Fisher Scientific (Waltham, MA, U.S.A.) 22–363-750 Fluriso VETone (Boise, ID, U.S.A) 502017 Isoflurane 250 mL, 2%–5% Fume absorber Labconco (Kansas City, MO, U.S.A.) - - General Protection Disposable SMS White Lab Coats Thermo Fisher Scientific (Waltham, MA, U.S.A.) 17–100-810A GraphPad Prism version 9 (La Jolla, CA, U.S.A.) Ice bucket - - any Microsoft Excel Microsoft (Redmond, WA, U.S.A.) Microsoft Word Microsoft (Redmond, WA, U.S.A.) Mouse cage - - any mouse cage holding at least 5 mice PrecisionGlide needles BD (Mississauga, ON, Canada) 329652 BD Slip Tip with PrecisionGlide Needle Insulin Syringes - 26 G x 3/8 0.45 mm x 10 mm Self-Sealing Sterilizing Pouch Fisher Scientific (Hampton, NY, U.S.A.) NC9241087 12.6 x 25.5 cm Small glass flask - - any, volume 25 mL sterile PBS Thermo Fisher Scientific (Waltham, MA, U.S.A.) 10010056 Stir bar Carl Roth GmbH & CO. KG X171.1 size according to volume of solution Stir plate Carl Roth GmbH & CO. KG AAN2.1 Syringe Luer-Lok BD (Mississauga, ON, Canada) 309628 1 mL syringe only Ultrasonic Cleaner, Heater/ Mechanical Timer Cole-Parmer (Vernon Hills, IL, U.S.A.) EW-08895–23 Bath sonicator - 0.5 gal, 115 V Vehicle solution - - depending on compound vehicle Vortex REAX Heidolph Instruments GmbH & Co. KG, Germany 541–10000-00 Open in a separate window Materials.

Techniques: Mass Measurement, Sterility

Journal: MethodsX

Article Title: An efficient ELISA protocol for measurement of SARS-CoV-2 spike-specific IgG in human plasma and serum samples

doi: 10.1016/j.mex.2024.102596

Figure Lengend Snippet:

Article Snippet: , Self-prepared buffers and solutions Stock solutions of PBSA and PBSA-S should be kept sterile and stored at 4 °C. • 0.1 M sodium hydrogen carbonate (pH 8.6) • 1 N sulfuric acid • PBS with 0.05 % Tween 20 (PBS-T5) • PBS with 5 % BSA (PBSA) • PBSA with 0.01 % Tween 20 (PBSA-T1) • PBSA with 20 % sheep serum (PBSA-S) Assay controls • Positive control: SARS-CoV-2 IgG high serum or plasma sample. If not available, a positive control can be generated by dilution of SARS-CoV-2 spike specific Ab, e.g. CR3022 (expression plasmid set used for production in FreeStyle 293-F cells: CAT# NR-53260, BEI Resources; ready-made Ab: CAT# NR-52481, BEI Resources, RRID:AB_2936472), in non-reactive serum or plasma. • Negative control: serum or plasma drawn from healthy donors before 2019. Equipment Utilizing a plate washer minimizes variation in assay results and is the preferred method over manual washing of ELISA plates using a multichannel pipette. • Class II biological safety cabinet, Thermo Scientific, CAT# 51025411 • Eppendorf Research plus pipettes: ○ 30 – 300 μL, 12-channel, CAT# 3125000060 ○ 2 – 20 μL, 1-channel, CAT# 3123000039 ○ 20 – 200 μL, 1-channel, CAT# 3123000055 ○ 100 – 1,000 μL, 1-channel, CAT# 3123000063 • Plate washer HydroSpeed, Tecan, CAT# 30054550, RRID:SCR_023457 • Plate reader Spark, Tecan, CAT# 30086376, RRID:SCR_021897 Consumables • Pipette tips with filter ○ 10-20 μl, TipOne, CAT# S1120-3710 ○ 20-200 μL, Biosphere, CAT# 70.1189.215 ○ 100-1000 μl, TipOne, CAT# S1122-1730 • Single use reservoir, Integra, CAT# 4312 • Plates: ○ 96-well plates, polystyrene, transparent, U-bottom, Greiner Bio-One, CAT# 650101 ○ 96-well ELISA plates, polystyrene, transparent, F-bottom, MaxiSorp, Nunc, Thermo Scientific, CAT# 442404 • 50 mL tubes, Greiner Bio-One, CAT# 227261 • EASYseal plate sealer, transparent, Grainer Bio-One, CAT# 676001 Software • SparkControl (version 3.1), Tecan, included with Plate washer • GraphPad Prism 9 (version 9.5.0), Dotmatics.

Techniques: Enzyme-linked Immunosorbent Assay, Software, Saline, Produced, Plasmid Preparation, Transfection, Sterility, Positive Control, Clinical Proteomics, Generated, Expressing, Negative Control, Transferring